Spectrophotometric methods for the simultaneous analysis of meclizine hydrochloride and pyridoxine hydrochloride in bulk drug and pharmaceutical formulations

Three new spectrophotometric procedures for the simultaneous determination of pyridoxine hydrochloride and meclezine hydrochloride are described. The first method depends on the application of simultaneous equation to resolve the interference due to spectral overlapping. The analytical signals were measured at 231 and 220 nm. Calibration graphs were established for 1 to 20 micro GmL-1 for pyridoxine hydrochloride and 0.5 to 10 micro GmL-1 for meclezine hydrochloride in binary mixture. In the second method, the determination of pyridoxine hydrochloride and meclezine hydrochloride was performed by measuring the absorbances at 290 and 235 nm in the simple absorbance spectra of their mixture. In third method a yellowish orange complex of pyridoxine hydrochloride was formed with ferric chloride, which absorbs in the visible region with

Validación del método analítico para la determinación de 3 vitaminas hidrosolubles en un suplemento vitamínico / Validation of the analytical method for the determination of 3 vitamins hydrosoluble in a vitamin supplement

Se presentan los resultados obtenidos en la validación de un método analítico por cromatografía líquida de alta resolución, para la determinación de tiamina mononitrato, piridoxina clorhidrato y nicotinamida en el suplemento nutricional neovitamin II, el cual se diseñó para separar las vitaminas entre sí, con la utilización de una columna RP-18 de 25 cm y un detector UV-Visible. Dicho método se empleó para el control de la calidad y la estabilidad de este producto. El método fue validado siguiendo una metodología de trabajo elaborada previamente en un Protocolo de Validación, donde se analizaron diferentes parámetros como son: linealidad, exactitud, precisión, selectividad, límites de detección y cuantificación, adecuación del sistema y estabilidad de las soluciones. Se obtuvieron resultados satisfactorios y se comprobó de esta forma la validez del método analítico

Factors affecting premenstrual tension syndrome [PMS] and the value of pyridoxine in its management

This study included 230 females with premenstrual tension syndrome [PMS], compared to 250 females forming the non-PMS group. The results showed that educational level, working condition, marital status, parity and contraceptive methods are all involved in the development of PMS. The diagnosed symptoms of premenstrual tension were more common among singles, students, secondary and highly educated females, and married women with one or two pregnancies, who use the IUD as a contraceptive method, and who is breast fed. These factors provide the patient criteria to allow for early intervention to control further complications and to carry out early measures for prevention. The double blind placebo-controlled part evaluated pyridoxine as a treatment for PMS. Pyridoxine did not relieve the symptoms to a statistically significant level, nor did it significantly affect the level of patients' serum prolactin, however, it improved some behavioral symptoms

Separaçäo de vitaminas pela eletroforese em papel / Separation of vitamins by paper electrophoresis

As vitaminas A (retino), B1 (tiamina), B6 (piridoxina), B12 (cianocobalamina), C (ácidoo ascórbico) e E (alfa-tocoferol), foram analisadas por eletroforese inidimensional em papel (qualitativo Klabin), usando como eletrólito soluçöes aquosas de carbonato de amônio 0,050, 0,075 e 0,100 M, sob um gradiente de potencial de 12,6V/cm. As melhores separaçöes ocorreram com o eletrólito nas concentraçöes 0,050 M (30 e 45 minutos) e 0,100 (45 e 60 minutos), quando 05 (cinco) vitaminas foram perfeitamente separadas: A (ou B6)-B1-B12-C e E